Sun, Yi and Wang, Xin and Chen, Guanghua and Song, Chengchao and Ma, Xinnan and Fu, Yutuo and Feng, Chao and Yan, Jinglong and Liu, Junyan (2020) miRNA-187-5p Regulates Osteoblastic Differentiation of Bone Marrow Mesenchymal Stem Cells in Mice by Targeting ICAM1. BioMed Research International, 2020. pp. 1-12. ISSN 2314-6133
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Abstract
Osteoporosis (OP) is a common bone metabolic disease, the process of which is fundamentally irreversible. Therefore, the investigation into osteoblastic differentiation of bone marrow mesenchymal stem cells (BMSCs) will provide more clues for OP treatment. In the present study, we found that microRNA-187-5p (miR-187-5p) played a key role on osteoblastic differentiation, which was significantly upregulated during osteogenic differentiation of BMSCs in mice. Moreover, overexpression of miR-187-5p suppressed osteoblastic differentiation of BMSCs through increasing alkaline phosphatase (ALP), matrix mineralization, and levels of Osterix (OSX), and osteopontin (OPN) as well as runt-related transcription factor 2 (Runx2) in vitro. The results in vivo indicated that the upregulation of miR-187-5p enhanced the efficacy of new bone formation in the heterotopic bone formation assay. Luciferase reporter assay and western blot analysis revealed that miR-187-5p was involved in osteogenesis by targeting intracellular adhesion molecule 1 (ICAM-1). Furthermore, ICAM-1 silence inhibited osteoblastic differentiation of BMSCs. Taken together, our results suggested for the first time that miR-187-5p may promote osteogenesis by targeting ICAM-1, and provided a possible therapeutic target for bone metabolic diseases.
Item Type: | Article |
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Subjects: | e-Archives > Medical Science |
Depositing User: | Managing Editor |
Date Deposited: | 01 Jun 2023 07:50 |
Last Modified: | 19 Jul 2024 07:41 |
URI: | http://ebooks.abclibraries.com/id/eprint/312 |