Akhtar, Tasleem and Ali, Ghazanfar and Shafi, Nuzhat and Akhtar, Wasim and Khan, Abdul Hameed and Latif, Zahid and Wali, Abdul and Ain-ul-Batool, Syeda and Khan, Abdul Rehman and Mumtaz, Sadia and Altaf, Syed Iftikhar and Khawaja, Sundus and Sadia, . and Khalid, Madiha and Rehman, Fazal Ur and Javid, Qudir and Fiore, Marco (2020) Sequencing and Characterization of Mitochondrial Protein-Coding Genes for Schizothorax niger (Cypriniformes: Cyprinidae) with Phylogenetic Consideration. BioMed Research International, 2020. pp. 1-13. ISSN 2314-6133
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Abstract
The present study was conducted to get more information about the genome and locate the taxonomic position of Schizothorax niger in Schizothoracinae through mitochondrial 13 protein-coding genes (PCGs). These PCGs for S. niger were found to be 11409 bps in length ranging from 165 (ATPase 8) to 1824 bps (NADH dehydrogenase subunit 5) and encode 3801 amino acids. In these PCGs, 4 genes overlap on the similar strands, while one shown on the opposite one: ATPase 6+8 and NADH dehydrogenase subunit 4+4L overlap by 7 nucleotides. Similarly, ND5-ND6 overlap by 4 nucleotides, while ATP6 and COIII overlap by 1 nucleotide. Similarly, four commonly used amino acids in S. niger were Leu (15.6 %), Ile (10.12 %), Thr (8.12 %), and Ala (8.7 %). The results presented that COII, COIII, NDI, ND4L, and Cytb had substantial amino acid conservation as compared to the COI gene. Through phylogenetic analysis, it was observed that S. niger is closely linked with S. progastus, S. labiatus, S. plagiostomus, and S. nepalensis with high bootstrap values. The present study provided more genomic data to know the diversity of the mitochondrial genome and its molecular evolution in Schizothoracinae.
Item Type: | Article |
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Subjects: | e-Archives > Medical Science |
Depositing User: | Managing Editor |
Date Deposited: | 13 Apr 2023 06:52 |
Last Modified: | 02 Sep 2024 12:15 |
URI: | http://ebooks.abclibraries.com/id/eprint/363 |