Pathology of Broiler Chicks Naturally Infected with Salmonella enteritidis (S. enteritidis) & Salmonella typhimurium (S. typhimurium) During an Outbreak in Sudan

Aims of the Study: To isolate and identify bacteria causing the disease, Characterize the bacterial isolates uses the automated machine vitek 2 compact, serotyping and Phage typing of bacterial isolates and study the histopathological finding due to the causative agents.

Place of Study: The samples collected from poultry farm included liver, intestine, kidney, spleen, heart, trachea and brain. Then transported immediately on ice to the Veterinary Research Institute, Soba for isolation, identification and characterization of bacteria.

Study Design: A total of eight thousand (8,000) broiler chicks, of the ‘Ross’ breed, were bought for commercial benefits in March 2014. Due to mortality that was started at the first day, postmortem was done to investigate the gross lesions and taking samples from liver, intestine, kidney, spleen, heart, trachea and brain.

Methodology: 52 samples from that organs were Isolated and identified according to bacteriological standard methods.

Automated system Vitek 2 compact was used to confirm and characterize the isolates.

Serotyping and phagetyping of isolates were done as further characterization.

Gross and histopathological lesions on different tissues were studied. All the histopathological pictures were found similar to those done by the previous researchers.