Antagonistic Compatibility of Streptomyces griseorubens, Gliocladium virens, and Trichoderma harzianum Againts Fusarium oxysporum Cause of Tomato Wilt Deseases

This research was intended to discover the compatibility of biological control agents (BCAs) Streptomyces griseorubens, Gliocladium virens, and Trichoderma harzianum againts Fusarium oxysporum f.sp. capsici in vitro and in vivo. Study was done in rainy season 2012-2013 at East Java-Indonesia. Study was a true experiment designed, using a completely randomized design (CRD), consist of three stages research. These stages were the compatibility of three biological agents in Potato Dextrose Agar (PDA) medium, antagonistic test of biological agents combinations of S. griseorubens, G. virens, and T. harzianum capable inhibited microbial pathogens F. oxysporum f.sp. lycopersici in the laboratory and at the screen house. Mixed of BCAs S. griseorubens, G. virens, and T. harzianum were compatible and effectively against F. oxysporum in Petri dishes and at screen house. Clear zona avarage of antibiosis of BCAs filtrat in Potato Glucose extract shown that the antibiosis from mixed of S. griseorubens and T. harzianum was higher than the antibiosis of other BCAs treatment. Plant infested with mixed of BCAs significantly protected plant tomato from F. oxysporum compared to the untreated control plants. Plant protection by BCAs mixed of T. harzianum with S. griseorubens was more pronounced than plant protection by mixed of S. griseorubens with G. virens and single BCAs.